CHO Cell Kab Muab Kev Pabcuam Customized
HEK293T (HEK293 hloov pauv) cell kab yog tib neeg embryonic raum cell kab muab los ntawm tib neeg embryo hauv xyoo 1970s.Nws yog siv nyob rau hauv ntau yam kev tshawb fawb chaw thiab yog ib qho cuab yeej tseem ceeb hauv kev kawm txog cov noob caj noob ces, cov qauv ntawm cov protein thiab kev ua haujlwm, kev hloov cov teeb liab, thiab kev tshawb nrhiav tshuaj.Cov hlwb yog ib qho yooj yim rau kis tau thiab feem ntau yog siv los kawm txog cov teebmeem ntawm ntau yam genetic manipulations, xws li overexpression los yog knockdown ntawm ntau yam genes, ntawm lub cell phenotype.Cov hlwb kuj tau siv rau hauv kev tshawb fawb ntawm qia cell biology, mob qog noj ntshav, thiab kev tiv thaiv kab mob.
Thawj Cell Culture
Thawj kab lis kev cai ntawm tes yog txheej txheem siv los loj hlob thiab tswj cov hlwb hauv vitro los ntawm ib lub xov tooj ntawm tes lossis ib pawg ntawm cov hlwb.Cov txheej txheem no yog siv los kawm txog tus cwj pwm thiab lub cev lub cev ntawm cov hlwb, thiab tuaj yeem siv rau ntau yam kev siv, suav nrog kev sim tshuaj, kev tshawb fawb kho mob, thiab kev kho mob ntawm tes.Cov kab lis kev cai tseem ceeb ntawm tes yog ua nyob rau hauv ib puag ncig tswj, feem ntau ntawm lub rooj zaum hauv chav kuaj, thiab tau txais kev txhawb nqa los ntawm ntau yam khoom tshwj xeeb thiab cov tshuaj reagents.Cov hlwb raug khaws cia kom ciaj sia los ntawm kev muab cov as-ham tsim nyog thiab tswj xyuas qhov kub thiab txias, pH, thiab qib oxygen.Cov hlwb tseem raug saib xyuas rau cov cim qhia ntawm kev ntxhov siab lossis kev sib kis, thiab cov kab lis kev cai raug tshuaj xyuas tas li rau txhua qhov kev hloov pauv hauv kev loj hlob lossis morphology.
Tib neeg Cell
Tib neeg lub cell yog qhov tseem ceeb tshaj plaws ntawm lub neej.Tib neeg lub cev yog tsim los ntawm trillions ntawm cov hlwb uas txhua tus muaj cov qauv tshwj xeeb thiab kev ua haujlwm.Cells yog lub tsev thaiv ntawm txhua yam muaj sia thiab muaj lub luag haujlwm rau kev loj hlob, metabolism, thiab lwm yam txheej txheem tseem ceeb.Cells yog tsim los ntawm ntau yam khoom, nrog rau cov proteins, DNA, carbohydrates, lipids, thiab organelles.
Secondary Cell Culture
Secondary cell kab lis kev cai yog cov txheej txheem ntawm culturing hlwb uas tau raug rho tawm thiab loj hlob nyob rau hauv lub laboratory ua ntej.Cells tuaj yeem loj hlob los ntawm cov ntaub so ntswg explants, dissociated nrog enzymes, los yog txawm cloned los ntawm ib lub hlwb.Secondary cell kab lis kev cai yog siv los nthuav cov kab ntawm tes, kawm txog kev coj tus cwj pwm ntawm tes, thiab tsim cov kev ntsuam xyuas ntawm tes.Cov cell hom siv nyob rau hauv cov kab lis kev cai theem nrab muaj xws li fibroblasts, endothelial hlwb, thiab cov leeg nqaij du.
